il22 3 utr Search Results


il22 3 utr  (Genecopoeia)
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Genecopoeia il22 3 utr
IL‐22 is the most likely candidate target of miR‐26 in CTCL. (A) Detection of potential miR‐26a target genes in MyLa. (Left): KEGG analysis of 127 downregulated genes in MyLa transduced with GFP‐miR‐26a lentivirus vector, compared with GFP‐empty lentivirus vector. (Right): List of miR‐26 targets of these 127 genes predicted using the indicated two programs in silico . (B) Sequence alignment of the mature miR‐26a/26b seed sequence and the 3′ untranslated region (UTR) of <t>IL22</t> . (C) RT‐qPCR analysis of IL22 and ELISA analysis of IL‐22 in MyLa and HH transduced with GFP‐empty (control) lentivirus vector or GFP‐miR‐26a lentivirus vector. EV, empty vector. (D) RT‐qPCR analysis of IL22 in MyLa transiently transduced with scramble control (Scr), miR‐26a, miR‐26b, or miR‐26a+miR‐26b (shown as “double”) for 24 h. (Right): Western blot analysis of IL‐22 in MyLa transiently transduced with Scr, miR‐26a, or miR‐26b for 48 h. Fold changes in IL‐22 levels are shown below the bands. Asterisks indicate statistical significance: *0.01 ≤ p < 0.05; **0.001 ≤ p < 0.01; *** p < 0.001; NS, not significant. Student t‐ test was used to test for significance. Bars represent the mean ± 95% confidence intervals of the three replicates
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IL‐22 is the most likely candidate target of miR‐26 in CTCL. (A) Detection of potential miR‐26a target genes in MyLa. (Left): KEGG analysis of 127 downregulated genes in MyLa transduced with GFP‐miR‐26a lentivirus vector, compared with GFP‐empty lentivirus vector. (Right): List of miR‐26 targets of these 127 genes predicted using the indicated two programs in silico . (B) Sequence alignment of the mature miR‐26a/26b seed sequence and the 3′ untranslated region (UTR) of IL22 . (C) RT‐qPCR analysis of IL22 and ELISA analysis of IL‐22 in MyLa and HH transduced with GFP‐empty (control) lentivirus vector or GFP‐miR‐26a lentivirus vector. EV, empty vector. (D) RT‐qPCR analysis of IL22 in MyLa transiently transduced with scramble control (Scr), miR‐26a, miR‐26b, or miR‐26a+miR‐26b (shown as “double”) for 24 h. (Right): Western blot analysis of IL‐22 in MyLa transiently transduced with Scr, miR‐26a, or miR‐26b for 48 h. Fold changes in IL‐22 levels are shown below the bands. Asterisks indicate statistical significance: *0.01 ≤ p < 0.05; **0.001 ≤ p < 0.01; *** p < 0.001; NS, not significant. Student t‐ test was used to test for significance. Bars represent the mean ± 95% confidence intervals of the three replicates

Journal: Cancer Science

Article Title: Downregulation of miR‐26 promotes invasion and metastasis via targeting interleukin‐22 in cutaneous T‐cell lymphoma

doi: 10.1111/cas.15296

Figure Lengend Snippet: IL‐22 is the most likely candidate target of miR‐26 in CTCL. (A) Detection of potential miR‐26a target genes in MyLa. (Left): KEGG analysis of 127 downregulated genes in MyLa transduced with GFP‐miR‐26a lentivirus vector, compared with GFP‐empty lentivirus vector. (Right): List of miR‐26 targets of these 127 genes predicted using the indicated two programs in silico . (B) Sequence alignment of the mature miR‐26a/26b seed sequence and the 3′ untranslated region (UTR) of IL22 . (C) RT‐qPCR analysis of IL22 and ELISA analysis of IL‐22 in MyLa and HH transduced with GFP‐empty (control) lentivirus vector or GFP‐miR‐26a lentivirus vector. EV, empty vector. (D) RT‐qPCR analysis of IL22 in MyLa transiently transduced with scramble control (Scr), miR‐26a, miR‐26b, or miR‐26a+miR‐26b (shown as “double”) for 24 h. (Right): Western blot analysis of IL‐22 in MyLa transiently transduced with Scr, miR‐26a, or miR‐26b for 48 h. Fold changes in IL‐22 levels are shown below the bands. Asterisks indicate statistical significance: *0.01 ≤ p < 0.05; **0.001 ≤ p < 0.01; *** p < 0.001; NS, not significant. Student t‐ test was used to test for significance. Bars represent the mean ± 95% confidence intervals of the three replicates

Article Snippet: We purchased an miTarget miRNA 3′UTR Target Clone (CmiT000001‐MT05) as the control vector and a vector containing the IL22 3′UTR (HmiT103252‐MT05) from GeneCopoeia (Rockville, MD, USA).

Techniques: Transduction, Plasmid Preparation, In Silico, Sequencing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Control, Western Blot

List of genes whose expression was reduced to one‐third or less after stable transduction with miR‐26a into MyLa compared with the control, and predicted to bind by the two prediction programs

Journal: Cancer Science

Article Title: Downregulation of miR‐26 promotes invasion and metastasis via targeting interleukin‐22 in cutaneous T‐cell lymphoma

doi: 10.1111/cas.15296

Figure Lengend Snippet: List of genes whose expression was reduced to one‐third or less after stable transduction with miR‐26a into MyLa compared with the control, and predicted to bind by the two prediction programs

Article Snippet: We purchased an miTarget miRNA 3′UTR Target Clone (CmiT000001‐MT05) as the control vector and a vector containing the IL22 3′UTR (HmiT103252‐MT05) from GeneCopoeia (Rockville, MD, USA).

Techniques: Expressing, Transduction, Control, Variant Assay, Sequencing

IL22 knockdown inhibits invasion and metastasis of CTCL cells in vivo . (A) RT‐qPCR analysis of IL22 in MyLa transduced with GFP‐scramble (Scr) or GFP‐shIL22 (#C, #D) lentivirus vector. (B) ELISA analysis of IL‐22 in MyLa transduced with GFP‐scramble (Scr) or GFP‐shIL22 (#C, #D) lentivirus vector. (C) Western blot analysis of IL‐22 in MyLa transduced with GFP‐scramble (Scr) or GFP‐shIL22 (#C, #D) lentivirus vector. (D) Proliferation assay of MyLa transduced with GFP‐scramble (Scr) or GFP‐shIL22 #D lentivirus vector. (E) Cell cycle analysis of MyLa transduced with GFP‐scramble (Scr) or GFP‐shIL22 #D lentivirus vector. (Left): Flow cytometry analysis with propidium iodide (PI) staining is shown. X‐axis: PI staining; Y‐axis: cell counts. (Right): DNA content (%) of subG1 ( N < 2n), G1 ( N = 2n), S (2n < N < 4n) and G2/M (N = 4n) phases are shown. (F) Migration assay of MyLa transduced with GFP‐scramble or GFP‐shIL22 #D lentivirus vector cultured for 24 h. (G) (Left): Photographs show the liver and spleen from NOG mouse after transplantation of MyLa transduced with GFP‐scramble or GFP‐shIL22 #D lentivirus vector (day 42). (Right): Liver weights of the two groups are shown. Asterisks indicate statistical significance: *0.01 ≤ p < 0.05; **0.001 ≤ p < 0.01; *** p < 0.001; NS, not significant. Student t ‐test was used to test for significance. Bars represent the mean ± 95% confidence interval of the three replicates

Journal: Cancer Science

Article Title: Downregulation of miR‐26 promotes invasion and metastasis via targeting interleukin‐22 in cutaneous T‐cell lymphoma

doi: 10.1111/cas.15296

Figure Lengend Snippet: IL22 knockdown inhibits invasion and metastasis of CTCL cells in vivo . (A) RT‐qPCR analysis of IL22 in MyLa transduced with GFP‐scramble (Scr) or GFP‐shIL22 (#C, #D) lentivirus vector. (B) ELISA analysis of IL‐22 in MyLa transduced with GFP‐scramble (Scr) or GFP‐shIL22 (#C, #D) lentivirus vector. (C) Western blot analysis of IL‐22 in MyLa transduced with GFP‐scramble (Scr) or GFP‐shIL22 (#C, #D) lentivirus vector. (D) Proliferation assay of MyLa transduced with GFP‐scramble (Scr) or GFP‐shIL22 #D lentivirus vector. (E) Cell cycle analysis of MyLa transduced with GFP‐scramble (Scr) or GFP‐shIL22 #D lentivirus vector. (Left): Flow cytometry analysis with propidium iodide (PI) staining is shown. X‐axis: PI staining; Y‐axis: cell counts. (Right): DNA content (%) of subG1 ( N < 2n), G1 ( N = 2n), S (2n < N < 4n) and G2/M (N = 4n) phases are shown. (F) Migration assay of MyLa transduced with GFP‐scramble or GFP‐shIL22 #D lentivirus vector cultured for 24 h. (G) (Left): Photographs show the liver and spleen from NOG mouse after transplantation of MyLa transduced with GFP‐scramble or GFP‐shIL22 #D lentivirus vector (day 42). (Right): Liver weights of the two groups are shown. Asterisks indicate statistical significance: *0.01 ≤ p < 0.05; **0.001 ≤ p < 0.01; *** p < 0.001; NS, not significant. Student t ‐test was used to test for significance. Bars represent the mean ± 95% confidence interval of the three replicates

Article Snippet: We purchased an miTarget miRNA 3′UTR Target Clone (CmiT000001‐MT05) as the control vector and a vector containing the IL22 3′UTR (HmiT103252‐MT05) from GeneCopoeia (Rockville, MD, USA).

Techniques: Knockdown, In Vivo, Quantitative RT-PCR, Transduction, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Western Blot, Proliferation Assay, Cell Cycle Assay, Flow Cytometry, Staining, Migration, Cell Culture, Transplantation Assay

miRNA and IL‐22 dysregulations involved in invasion and metastasis of advanced CTCL. (A) miR‐26a‐IL22 axis involved in CTCL invasion and metastasis from the present study. (B) Schema of miRNA dysregulations involved in CTCL invasion and metastasis from our present and previous studies. (C) Two miRNA dysregulations with anti‐apoptosis ability and involved in invasion/metastasis in CTCL

Journal: Cancer Science

Article Title: Downregulation of miR‐26 promotes invasion and metastasis via targeting interleukin‐22 in cutaneous T‐cell lymphoma

doi: 10.1111/cas.15296

Figure Lengend Snippet: miRNA and IL‐22 dysregulations involved in invasion and metastasis of advanced CTCL. (A) miR‐26a‐IL22 axis involved in CTCL invasion and metastasis from the present study. (B) Schema of miRNA dysregulations involved in CTCL invasion and metastasis from our present and previous studies. (C) Two miRNA dysregulations with anti‐apoptosis ability and involved in invasion/metastasis in CTCL

Article Snippet: We purchased an miTarget miRNA 3′UTR Target Clone (CmiT000001‐MT05) as the control vector and a vector containing the IL22 3′UTR (HmiT103252‐MT05) from GeneCopoeia (Rockville, MD, USA).

Techniques: